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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
11/06/2019 |
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Data da última atualização: |
06/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FALAVIGNA, V. da S.; MALABARBA, J.; SILVEIRA, C. P.; BUFFON, V.; MARIATH, J. E. de A.; PASQUALI, G.; MARGIS-PINHEIRO, M.; REVERS, L. F. |
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Afiliação: |
Vítor da Silveira Falavigna, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, Brazil; Jaiana Malabarba, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, BraziL; Carolina Pereira Silveira, Embrapa Uva e Vinho, Rua Livramento, 515, P.O. Box 130, Bento Gonçalves, RS 95701-008, Brazil; VANESSA BUFFON, CNPUV; Jorge Ernesto de Araújo Mariath, Laboratory of Plant Anatomy, Botany Department, UFRGS, Porto Alegre 91501-970, Brazil; Giancarlo Pasquali, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, Brazil; Márcia Margis?Pinheiro, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, Brazil; LUIS FERNANDO REVERS, CNPUV. |
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Título: |
Characterization of the nucellus-specific dehydrin MdoDHN11 demonstrates its involvement in the tolerance to water deficit. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Plant Cell Reports [online], 2019. |
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DOI: |
10.1007/s00299-019-02428-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Key message MdoDHN11 acts in the nucellus layer to protect the embryo and the endosperm from limited water availability during apple seed development.AbstractDehydrins (DHNs) are protective proteins related to several plant developmental responses that involve dehy-dration such as seed desiccation and abiotic stresses. In apple (Malus × domestica Borkh.), the seed-specific MdoDHN11was suggested to play important roles against dehydration during seed development. However, this hypothesis has not yet been evaluated. Within this context, several experiments were performed to functionally characterize MdoDHN11. In situ hybridization analysis during apple seed development showed that MdoDHN11 expression is confined to a maternal tissue called nucellus, a central mass of parenchyma between the endosperm and the testa. The MdoDHN11 protein was local-ized in the cytosol and nucleus. Finally, transgenic Arabidopsis plants expressing MdoDHN11 were generated and exposed to a severe water-deficit stress, aiming to mimic a situation that can occurs during seed development. All transgenic lines showed increased tolerance to water deficit in relation to wild-type plants. Taken together, our results provide evidences that MdoDHN11 plays important roles during apple seed development by protecting the embryo and the endosperm from limited water availability, and the mechanism of action probably involves the interaction of MdoDHN11 with proteins and other components in the cell.Keywords Apple · Dehydrin · Seed development · Transgenic expression · Water-deficit stress MenosAbstract Key message MdoDHN11 acts in the nucellus layer to protect the embryo and the endosperm from limited water availability during apple seed development.AbstractDehydrins (DHNs) are protective proteins related to several plant developmental responses that involve dehy-dration such as seed desiccation and abiotic stresses. In apple (Malus × domestica Borkh.), the seed-specific MdoDHN11was suggested to play important roles against dehydration during seed development. However, this hypothesis has not yet been evaluated. Within this context, several experiments were performed to functionally characterize MdoDHN11. In situ hybridization analysis during apple seed development showed that MdoDHN11 expression is confined to a maternal tissue called nucellus, a central mass of parenchyma between the endosperm and the testa. The MdoDHN11 protein was local-ized in the cytosol and nucleus. Finally, transgenic Arabidopsis plants expressing MdoDHN11 were generated and exposed to a severe water-deficit stress, aiming to mimic a situation that can occurs during seed development. All transgenic lines showed increased tolerance to water deficit in relation to wild-type plants. Taken together, our results provide evidences that MdoDHN11 plays important roles during apple seed development by protecting the embryo and the endosperm from limited water availability, and the mechanism of action probably involves the interaction of MdoDHN11 with proteins and other components in the cell.Keywor... Mostrar Tudo |
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Palavras-Chave: |
Transgenic expression; Water-deficit stress. |
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Thesaurus Nal: |
Apples; Dehydrins; Seed development. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198444/1/Falavigna2019-Article-CharacterizationOfTheNucellus-.pdf
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Marc: |
LEADER 02425naa a2200277 a 4500
001 2109788
005 2020-01-06
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1007/s00299-019-02428-8$2DOI
100 1 $aFALAVIGNA, V. da S.
245 $aCharacterization of the nucellus-specific dehydrin MdoDHN11 demonstrates its involvement in the tolerance to water deficit.$h[electronic resource]
260 $c2019
520 $aAbstract Key message MdoDHN11 acts in the nucellus layer to protect the embryo and the endosperm from limited water availability during apple seed development.AbstractDehydrins (DHNs) are protective proteins related to several plant developmental responses that involve dehy-dration such as seed desiccation and abiotic stresses. In apple (Malus × domestica Borkh.), the seed-specific MdoDHN11was suggested to play important roles against dehydration during seed development. However, this hypothesis has not yet been evaluated. Within this context, several experiments were performed to functionally characterize MdoDHN11. In situ hybridization analysis during apple seed development showed that MdoDHN11 expression is confined to a maternal tissue called nucellus, a central mass of parenchyma between the endosperm and the testa. The MdoDHN11 protein was local-ized in the cytosol and nucleus. Finally, transgenic Arabidopsis plants expressing MdoDHN11 were generated and exposed to a severe water-deficit stress, aiming to mimic a situation that can occurs during seed development. All transgenic lines showed increased tolerance to water deficit in relation to wild-type plants. Taken together, our results provide evidences that MdoDHN11 plays important roles during apple seed development by protecting the embryo and the endosperm from limited water availability, and the mechanism of action probably involves the interaction of MdoDHN11 with proteins and other components in the cell.Keywords Apple · Dehydrin · Seed development · Transgenic expression · Water-deficit stress
650 $aApples
650 $aDehydrins
650 $aSeed development
653 $aTransgenic expression
653 $aWater-deficit stress
700 1 $aMALABARBA, J.
700 1 $aSILVEIRA, C. P.
700 1 $aBUFFON, V.
700 1 $aMARIATH, J. E. de A.
700 1 $aPASQUALI, G.
700 1 $aMARGIS-PINHEIRO, M.
700 1 $aREVERS, L. F.
773 $tPlant Cell Reports [online], 2019.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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| Registros recuperados : 15 | |
| 1. |  | SILVA, M. S.; NASCIMENTO, C. C. H. C.; OLIVEIRA, J. F. F.; NASCIMENTO, S. F.; VASCONCELOS, S. D. D. de; NOGUEIRA, R. I.; STEPHENS, P. R. S.; DIRÉ, G. F.; BARRETO, A. S. Evaluation of the preliminary phytochemical profile and antioxidant activity of an aqueous extract processed from Myrciaracemosa leaves. Merit Research Journal of Medicine and Medical Sciences , v. 6 , n. 2, p. 61-67, Feb. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 2. | | SILVA, E. J.; NASCIMENTO, C. C. H. C.; NASCIMENTO, S. F.; VASCONCELOS, S. D. D. de; NOGUEIRA, R. I.; STEPHENS, P. R. S.; BARRETO, A. S.; DIRÉ, G. F. Study of the Biological Effects of Aqueous Extracts of Schinus terebinthifolius Raddi, on the survival fraction of mutant and wild strains of Escherichia coli. Journal of Analytical & Pharmaceutical Research, v. 7 , n. 2, p. 268-269, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 3. | | PENTEADO, F. S. C.; NASCIMENTO, C. C. H. C.; VASCONCELOS, S. D. D. de; CAMACHO, A. C. L. F.; NASCIMENTO, S. F.; NOGUEIRA, R. I.; BARRETO, A. S.; DIRÉ, G. F. Evaluation of the biological effects of aqueous extracts from the leaf of Bauhinia Blakeana Dunn. World Journal of Pharmaceutical and Medical Research, v. 3, n. 8, p. 1-8, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 4. | | SILVA, M. S.; NASCIMENTO, C. C. H. C.; CAMACHO, A. C. L. F.; SOUZA, M. C.; OLIVEIRA, J. F. F.; NASCIMENTO, S. F.; VASCONCELOS, S. D. D. de; NOGUEIRA, R. I.; DIRÉ, G. F.; BARRETO, A. S. Study of the biological effects of an aqueous Myrcia Racemosa extract on bacterial cultures in the presence and absence of stannous chloride solution. European Journal of Pharmaceutical and Medical Research, v. 4, n. 11, p. 16-24, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 5. | | NASCIMENTO, C. C. H. C.; AZEVEDO, L. A. C.; CAMACHO, A. C. L. F.; OLIVEIRA, J. F. F.; GOMES, M. L.; NASCIMENTO, S. F.; VASCONCELOS, S. D. D. de; NOGUEIRA, R. I.; BARRETO, A. S.; DIRÉ, G. F. Analysis of hypoglycemic effect of an aqueous extract of Costus spicatus on F1 mice subjected to hyperglycemic diet. International Journal of Advanced Research in Biological Science, v. 3, n. 2, p. 99-107, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 6. | | NASCIMENTO, C. C. H. C.; VASCONCELOS, S. D. D. de; NASCIMENTO, S. F.; OLIVEIRA, J. F. F.; NOGUEIRA, R. I.; BARRETO, A. S.; DIRÉ, G. F. Analysis of measurement of weight of animals treated with a freeze-dried aqueous extract of costus spicatus. World Journal of Pharmaceutical and Medical Research, v. 2, n. 4, p. 190-195, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 7. | | RODRIGUES, V. G.; NASCIMENTO, C. C. H. C.; CAMACHO, A. C. L. F.; OLIVEIRA, J. F. F.; GOMES, M. L.; VASCONCELOS, S. D. D. de; NOGUEIRA, R. I.; BARRETO, A. S.; DIRÉ, G. F. Electrically voltage profile of the study samples of an aqueous extract of Syzygium jambolanum. International Journal of Life-Sciences Scientific Research, v. 2, n. 4, p. 438-441, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 8. | | NASCIMENTO, C. C. H. C; VASCONCELOS, S. D. D. de; CAMACHO, A. C. L. F.; NASCIMENTO, S. F.; Oliveira, J. F. F.; NOGUEIRA, R. I.; BARRETO, A. S.; DIRÉ, G. F. A literature review on the medicinal properties and toxicological profile of Costus spicatus plant. Research Journal of Life Sciences, Bioinformatics, Pharmaceutical and Chemical Sciences, v. 2, n. 2, p. 56-62, ago., 2016. 2454-6348| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 9. | | NASCIMENTO, C. C. H. C.; ARMANDA-DIAS, L. C.; AZEVEDO, L. A. C.; CAMACHO, A. C. L. F.; OLIVEIRA, J. F. F.; GOMES, M. L.; NASCIMENTO, S. F.; VASCONCELOS, S. D. D. de; NOGUEIRA, R. I.; BARRETO, A. S.; DIRÉ, G. F. Study of the biological effect of an aqueous extract from a medicinal plant (Costus spicatus) F1 mice treated with hyperglycaemic diet. International Journal of Research in Biosciences, v. 5, n. 2, p. 1-9, apr., 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 10. | | SANTOS, J. L.; CRUZ, G. C. S.; NASCIMENTO, C. C. H. C.; CAMACHO, A. C. L. F.; OLIVEIRA, J. F. F.; GOMES, M. L.; AZEVEDO, L. A. C.; PINTO, P. R.; NASCIMENTO, S. F.; VASCONCELOS, S. D. D. de; NOGUEIRA, R. I.; BARRETO, A. S.; DIRÉ, G. F. Study of the biological effects of an essential oil processed from leaves of Eugenia astringens cambess specimens. (Myrtaceae). European Journal of Biomedical and Pharmaceutical sciences, v. 3, n. 1, p. 36-41, jan., 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 11. | | RODRIGUES, V. G.; NASCIMENTO, C. C. H. C.; VASCONCELOS, S. D. D. de; NASCIMENTO, S. F.; OLIVEIRA, J. F. F.; NOGUEIRA, R. I.; BARRETO, A. S.; DIRÉ, G. F. Study of the change of the electrical conductivity of a lyophilized aqueous extract of syzygium jambolanum under different treatment conditions. European Journal of Pharmaceutical and Medical Research, v. 3, n. 8, p. 631-336, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 12. | | CARLOS, A. L. M.; DIRÉ, G. F.; NASCIMENTO, C. C. H. C.; NASCIMENTO, S. F.; VASCONCELOS, S. D. D. de; CAMACHO, A. C. L. F.; OLIVEIRA, J. F. F.; NOGUEIRA, R. I.; BARRETO, A. S.; PINTO, P. R. Study of the synthesis and characterization of a poly (ethylene succinate) biodegradable polyester with possible application as encapsulating matriz of essential oil of lemongrass. European Journal of Biomedical and Pharmaceutical Sciences, v. 3, n. 3, p. 7-22, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 13. | | PEIXOTO, A. G.; VIEIRA, T. O.; MELO, B. T. A.; NASCIMENTO, C. C. H. C.; AZEVEDO, L. A. C.; CAMACHO, A. C. L. F.; OLIVEIRA, J. F. F.; GOMES, M. L.; BARROS NETO, L. C. de; NASCIMENTO, S. F.; NOGUEIRA, R.; BARRETO, A. S.; DIRÉ, G. F. Identification and characterization of the phenolic profile of a hydroalcoholic extract of Punica Granatum. International Journal Of Medical Science And Clinical Inventions, v. 2, n. 4, 2015, p. 821-836.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 14. | | PEIXOTO, A. G.; VIEIRA, T. O.; MELO, B. T. A.; NASCIMENTO, C. C. H. C.; AZEVEDO, L. A. C.; CAMACHO, A. C. L. F.; OLIVEIRA, J. F. F.; GOMES, M. L.; BARROS NETO, L. C. de; NASCIMENTO, S. F.; NOGUEIRA, R. I.; BARRETO, A. S.; DIRÉ, G. F. Study of the biological effects of a hydroalcoholic extract of PUNICA GRANATUM (POMEGRANATE). International Journal of Current Research in Chemistry and Pharmaceutical Sciences, v. 2, n. 6, p. 44-58, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 15. | | PEIXOTO, A. G.; NASCIMENTO, C. C. H. C.; AZEVEDO, L. A. C.; PAIM, D. R. S. F.; NOGUEIRA, R. I.; BARRETO, A. S.; DIRÉ, G. F. Chemical analysis of biological effects of a hydroalcoholic extract Punica granatum (Pomegranate). Innovative Journal of Medical and Health Science, v. 4, n. 6, p. 172-179, nov./dec. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| Registros recuperados : 15 | |
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